WebJan 25, 2012 · A, Analysis of mouse whole-brain mRNA by qPCR shows a dramatic change in the ratio of synaptoneurosomal (S) to total homogenate (H) Fabp7 mRNA compared with GAPDH and β-actin mRNA across the day. B, Analysis of mouse brain protein also shows a time-of-day cycling of Fabp7 enrichment in the ratio of synaptoneurosomal (S) to total … WebSep 29, 2012 · The yield (%) is calculated from the total activity (units) at each step divided by the total activity (units) in the homogenate, multiplied by 100. The yield can also be calculated on a “per step” basis by dividing the total activity after that step by the total activity before that step and multiplying by 100.
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We compared SN and TH transcriptomes from mouse amygdala and detected 17 514 and 18 318 transcripts in the SN and TH microarrays, respectively, with a high overlap of detected transcripts (17 265). We studied the expression levels using principal component analysis and found a distinct clustering of the two … See more We used WGCNA32 to group genes into modules that have strong co-varying (similar) patterns of expression across the sample set. Hierarchical clustering showed that the SN preparation contains groups of mRNAs that … See more A cell-type-specific enrichment analysis was performed for the alcohol-responsive mRNAs using neuronal, astrocytic, oligodendrocyte, microglial, GABA and glutamate gene lists (see Methods for details). The … See more WebTotal recoveries of enzyme activities and protein were all between 95 and 100% as compared to the unfractionated homogenate. The latter hydrolysed glucose-6-phosphate … how to repair a metal garden hose
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WebMar 21, 2024 · They periodically analyzed samples to determine the total viable count and the pathogen population along 56 days of storage. ... the study found that packaging materials most and least conducive to the survival of L. monocytogenes in cheese homogenate were greaseproof paper and polyethylene-coated nylon, respectively. WebJan 6, 2024 · Synaptosomes isolation and characterization. a Representation of synaptosomal particles with pre- and post-synaptic areas evidenced.b Synaptosomal band (red arrow) collected at sucrose interface after ultracentrifugation in sucrose fractionation.c Western blot of proteins extracted from total homogenate (Total) and synaptosomal … Web12 hours ago · Fresh bamboo shoots (Chimonobambusa quadrangularis) are subjected to senescence (e.g., lignification and browning) during postharvest storage. This study investigated the effects of 1-MCP and SO2 treatment on bamboo shoot senescence and its regulation mechanism in order to extend bamboo shoot storage time. 1-MCP and SO2 … how to repair a microwave