WebMonoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 cells) and cells from BALB/c mice immunized with a synthetic peptide YSPTS(O-GlcNAc)PSK conjugated to KLH.This antibody recognizes O-GlcNAc in β-O-glycosidic … WebIn 2008, the Okajima group originally discovered the O-GlcNAc modification on Drosophila Notch EGF20 by detecting O-glycans on the ECD of Drosophila Notch using an antibody that recognizes O-GlcNAc (CTD110.6) . Later, O-GlcNAc modifications of the EGF repeats in Drosophila Delta, Serrate, and Dumpy were subsequently discovered [86,87].
Alexa Fluor 594 anti-O-GlcNAc Antibody anti-O-GlcNAc - CTD110.6 …
WebSpecificity. Clone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The … WebClone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The presence of GlcNAc, but not GalNAc, abolished the detection of the target modification. Clone CTD110.6 did not recognize peptides with unmodified serine and threonine residues (Comer, F.I., et al. (2001). care of wool rugs
Adenovirus Type 11 Uses CD46 as a Cellular Receptor - PMC
WebDec 9, 2016 · The RL2 epitope was detected on Sp1 during 2DG treatment, and the level was transiently increased at 24 h. In contrast, the CTD110.6 epitope became detectable on Sp1 over 72 h after 2DG treatment, and then the other proteins containing CTD110.6 epitopes also appeared in the cell lysates and the anti-Sp1 antibody precipitates. WebO-GlcNAc antibody (mAb) (Clone CTD110.6) for use in Immunoprecipitation (IP) ... This antibody recognizes single O-GlcNAc residues in b-O-glycosidic linkage to serine and … WebMay 18, 2024 · 3. Incubate the blots with CTD110.6 (diluted to 1 μg/ml in antibody dilution buffer), in duplicate, with and without 100 mM GlcNAc, overnight at 4°C. An antibody that has been antigen purified using a GlcNAc-agarose column yields higher-quality blots. Commercially available CTD110.6 is purified by ammonium sulfate precipitation. care of you the fifthguys lyrics