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Bsp73as

WebJan 11, 2024 · a BSp73AS and BspASs6 cells were transfected with the ErbB1 receptor expression vector or a control vector by means of the retroviral ecopack 2–293 system as described in Material and Methods ... WebMigrationwasquantifiedaccordingtoManual_trackingplugin (F.P.Cordelie´re,CentredeRecherchedel’InstituteCurie)run-ningintheopen-sourcesoftwareImageJ(NIH).Pathlengthof

CD44v6CoordinatesTumorMatrix-triggeredMotilityand …

Web3/4 hp, 115V submersible pump. 115 Volt operation allows the pump to be plugged into a standard outlet. (GFCI is recommended). Ideal for draining basements, window wells, … WebJan 29, 2024 · TEX were derived from non-metastatic BSp73AS (AS) or metastatic BSp73ASML (ASML) rat tumor lines transfected with Tspan8 (AS-Tspan8) or Tspan8-shRNA (ASML-Tspan8kd). mRNA was analyzed by deep sequencing and miRNA by array analysis of EC and Fb before and after coculture with TEX. EC and Fb responded more … herpes infection of the throat https://fareastrising.com

A New Variant of Glycoprotein CD44 Confers …

WebThe rat cell lines BSp73AS (AS),26 C4.4A cDNA–transfected BSp73AS (AS-1B1),18 BSp73ASML (ASML),26 Progressor27 and 804G28 were grown in RPMI medium supplemented with 10% FCS. The HEK-293 EBNA cell line, a kind gift of Dr. E. Tamm (Department of Anatomy, University of Erlangen, Erlangen), was grown in Iscove’s … WebJun 1, 2006 · BSp73AS-D6.1A-, but also BSp73AS-induced angiogenesis. in vivo and in vitro. This finding sugg ested the existence of. an additional antibody target that has been identified as prolif- WebDec 10, 2009 · Transfection of the BSp73AS cells and BSp73ASs6 cells was performed by electroporation. In brief, 3 × 10 6 cells were mixed with 5 μg of vector DNA on ice; the electroporation was performed in a 4-mm electroporation cuvette using a Gene Pulser (Bio-Rad) at 250 μF, 0.28 kV. Prewarmed medium containing serum was added, and the cells … maxwell alves sdt

Cells Free Full-Text Tspan8-Tumor Extracellular Vesicle-Induced ...

Category:Surface staining of Iransfectants. BSp73AS. BSp73ASML,.

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Bsp73as

Changes in adhesive properties of tumor cells do not ... - PubMed

WebJul 1, 1996 · Transfection of a surface-bound hyaluronidase into metastasizing BSp73AS cells bearing variant CD44 efficiently ablated the ability of these cells to bind to HA. … WebMar 1, 2009 · HEK cells and the rat pancreatic tumor line BSp73AS, transfected with (mutated) EpCAM and claudin-7 cDNA, revealed that the association of both molecules severely alters the functional activity of EpCAM. Claudin-7–associated EpCAM is recruited into tetraspanin-enriched membrane microdomains (TEM). The TEM-located claudin-7 …

Bsp73as

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WebMar 15, 1993 · The variant sequences are inserted just outside the transmembrane region of the molecules. The two most abundant variants have 162 and 85 extra amino acids, respectively. When individually expressed, these suffice to establish metastatic properties in the nonmetastatic tumor cell line BSp73AS, as assayed by the spontaneous metastasis … WebCheck out P2073A & P2073A SQ at Unistrut.us. Materials & Finishes - Standard: . Base Metal: Steel conforms to ASTM A1011 SS GR 33, A1011 HSLAS GR 45, A36, A575, …

WebJun 1, 2006 · BSp73AS-D6.1A-, but also BSp73AS-induced angiogenesis. in vivo and in vitro. This finding sugg ested the existence of. an additional antibody target that has been identified as prolif- WebJul 15, 2004 · In BSp73AS-14 cells too, most CD44v4-v7 clusters were stained by phalloidin-FITC. In BSp73AS cells, hardly any colocalization of CD44s with actin bundles …

Webbsp73as. BSp73ASML, BSp73ASpSVMeta-l, and BSp73ASpSVMeta-2 cells were washed twice with PBS, fixed in 1% paraformaldehyde. and incu bated with M Ab 1.1ASML at …

WebNov 1, 2003 · (A) BSp73AS-β4, BSp73AS-D6.1A, BSp73AS-db, BSp73ASML and Progressor cells were labeled with [3 H]thymidine and seeded on BSA- or recombinant …

WebJan 1, 1993 · Expression of a splice variant of CD44, recognised by the monoclonal antibody (Mab) 1.1ASML, confers metastatic potential to non-metastasising tumour cells (Cell1991, 65, 13–24).To explore whether the metastasis-associated variant of CD44 (CD44v) is expressed under physiological conditions, tissues of newborn and adult rats … maxwell amenityWebWe show that transfection of this CD44 variant into BSp73AS cells increases the HA- binding capacity of the cells in a dose-dependent manner. Transfection of the same CD44 variant isoform into BDX2 cells also conferred strong HA-binding properties on these cells, but was insufficient to cause them to metastasize. maxwell alves solicitorsWebDec 20, 2005 · To answer the question, metastasis formation was evaluated in the nonmetastasizing rat pancreatic adenocarcinoma subline BSp73AS (AS), which was stably transfected with SMAGP cDNA (AS-SMAGP). Cytoplasmic SMAGP expression promoted cell agglomeration, but inhibited tumor cell proliferation, adhesion to and migration … maxwell aluminum compact backpack chairWebDec 1, 2011 · CD44v6-induced expression in nonmetastatic BSp73AS cells induced lung metastasis when injected into syngeneic rats, yet treatment with the CD44v6 peptides completely abolished metastatic dissemination . Similarly, the CD44v6 peptide was used to inhibit the cooperation of CD44v6 with Met and VEGFR2 in endothelial cells . The v6 … herpes infection of the fingerWebApr 1, 1993 · BSp73AS cells results in synthesis of a protein with an apparent. molecular weight of 115,000 as the modified form and of 70.000 after. tunicamycin treatment (Fig. 7. Lanes B and D), while the ... maxwell alvesWebBuy genuine Unistrut P2073A, P2073ASQ post base fitting online and save with free shipping. Compatible with all 1-5/8" metal framing strut. In stock for immediate shipment maxwell ammons morehead stateWebFits various Briggs & Stratton models. This product is manufactured in United States. This is a replacement part. Part Number 1727873ASM. This is an O.E.M. part. +. +. $109.17. … maxwell a. miller attorney